BioMediTech Research Infrastructure

Flow Cytometry

Introduction

Flow cytometry is a fluorescence-based method in which fluorescent-stained cells are radiated with a laser and the strength of the emission by the cell is measured. Cells are analyzed and classified on the basis of bothfluorescence and light emission. This yields information on cell size and structure and on the cell receptors. The cell-sorting cytometer is known as the FACS (Fluorescence Activated Cell Sorter).

Flow Cytometry facilities are currently under reorganization.

Equipment

Accuri C6 Flow Cytometer

An industry-standard, sheath-focused core facilitates high performance while enabling event rates of up to 10,000 events/second and sample concentrations over 5×107 cells/ml. The device measures the volume pulled from your sample so that you can calculate cell concentration. It has a fully digital data collection system, allowing complete flexibility to display and analyze data post-collection, without permanent alteration of your original data file.

Equipped with a blue and a red laser, two scatter detectors, and four fluorescence detectors with interference filters optimized for the detection of FITC, PE, PerCP-Cy5.5 and APC.

Works with the same reagents you currently use on the market- leading flow cytometers.

C6 fluidics system is non-pressurized enabling any brand of sample tube that is 12×75 mm or smaller to be used. This includes microcentrifuge tubes and tubes made of polypropylene or polystyrene.

CFlow® files can be exported in FCS 3.0 format for seamless importation of your data into most 3rd party flow cytometry software programs including FCS Express and FlowJo.

Usage of Accuri is allowed only after the first free introduction to the instruments. Further tutorials are possible with an extra price. All solutions except your sample suspension buffer are included. All researchers must fill the log book with complete information of the billing address to be used for charges. From UTA personnel your name and group info (and possible fund number) is sufficient.

Instruction Manuals

7820011B CFlow Guide (due to a big file size divided into three parts: part 1, part 2, part 3) (pdf)

7820023A C6 Start-up and Shutdown Card (pdf)

Accuri C6 Flow Cytometer Instrument Manual (pdf)

QuickStart Guide Analyze (pdf)

QuickStart Guide Collect (pdf)

QuickStart Guide Statistics (pdf)

Prices

Accuri 6 Flow Cytometer

Do an advance reservation for the instrument. Please be aware that if you cancel your reservation later than one working day before your reservation, you are still charged for the reserved time. If you need to cancel your reservation, please do so as early as possible.

User Service Price
Academic researchers Instrument usage
Advanced tutorials
8 €/h
26 €/h
Others Instrument usage
Advanced tutorials
16 €/h
60 €/h

All the bills will be topped by overheads and VAT when applicable.

Contacts

Laura Kummola
laura.kummola(at)uta.fi
Tel: +358 50 318 6352
Room: ARVO F354

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