BioMediTech Research Infrastructure

Electrophysiological Measurements

Tampere Facility of Electrophysiological Measurements offers state-of-the-art equipment for electrophysiological recordings, fast fluorescence imaging, and impedance and potential measurements for life scientists as well as biomedical engineers.

Introduction

Facility includes patch clamp units, fast fluorescence imaging units and several microelectrode array (MEA) formats. These three methods can be combined in one of the setups. In addition, facility includes transretinal electro-retinogram (ERG) system. Also, additional in-house built measurement systems can be combined with aforementioned systems.

All of the systems are located in rooms specifically designed for electrophysiological measurements. The laboratory is equipped with dedicated cell culture room to enable repeatable measurements in high purity, contrast phase and fluorescence microscopes for additional sample imaging etc. It also contains work bench area where other experimental designs and test experiments can be performed in a designated incubator. Data analysis stations are available for detailed fast imaging and patch clamp analysis in the office premises.

Current applications areas:

  • patch clamp, fast fluorescence imaging, and MEA with human embryonic and induced pluripotent stem cells derived neural cells and cardiomyocytes
  • patch clamp and fast fluorescence imaging with human pluripotent stem cell derived retinal epithelial pigmented cells
  • combined patch clamp and fast fluorescence imaging human pluripotent stem cell derived retinal epithelial pigmented cells and cardiomyocytes
  • combined fast fluorescence imaging and MEA with human pluripotent stem cell derived neural cells
  • fast fluorescence imaging with human retinal pigment epithelial cell line (ARPE-19 cells)
  • MEA with ex vivo retina (mouse/rat origin)

Facility is equipped and designed in collaboration with BMT (UTA) and BMT (TUT) research groups.

ACKNOWLEDGEMENT

All the users of Tampere Facility of Electrophysiological Measurements services are obligated to acknowledge the facility in publications:

“The authors acknowledge Tampere Facility of Electrophysiological Measurements for their service.”

Equipment

Fast fluorescence imaging and Patch clamp systems

Fast fluorescence imaging and patch clamp system

Patch Clamp Measurements

Patch clamp measurements of human cardiomyocytes. In this system, combined patch clamp, fast fluorescence imaging and MEA measurements can be performed. (Photo: Jonne Renvall/University of Tampere)

Till Photonics Imaging Control Unit and Polychrome V

Molecular devices Axopatch 200B amplifier
Molecular devices Digidata 1440A Low-Noise data acquisition system
L&N Micromanipulators and motorized shifting portal SM5-9
NPI SEC-05X amplifiers for dual patch mode

Olympus inverted microscope IX71
https://www.olympus-lifescience.com/data/olympusmicro/brochures/pdfs/ix71.pdf?rev=EABE

Gravitation based perfusion

Patch Clamp system

Stand-alone Patch Clamp Unit

Stand-alone patch clamp unit. (Photo: Jonne Renvall/University of Tampere)

Molecular devices Axopatch 200B amplifier

Molecular devices Digidata 1440A Low-Noise data acquisition system

Scientifica Patchstar micromanipulators
http://www.scientifica.uk.com/products/scientifica-patchstar-micromanipulator

Olympus inverted microscope IX73
https://www.olympus-lifescience.com/en/microscopes/inverted/ix73/

Gravitation based perfusion

Fast fluorescence imaging system

Stand-alone Fast Fluorescence Imaging Unit

Stand-alone fast fluorescence imaging unit. (Photo: Jonne Renvall/University of Tampere)

Zeiss Axio Observer A1
https://www.zeiss.com/microscopy/int/products/light-microscopes/axio-observer-for-biology.html

Sutter DG-4
https://www.sutter.com/IMAGING/lambdadg4.html

Gravitation based perfusion

MEA systems

Axion Biosystems Maestro

Measurement of neuronal network activity

Measurement of neuronal network activity. (Photo: Jonne Renvall/University of Tampere)

Features:

  • Multi-well microelectrode array with 768-channel measurement platform for high throughput measurements. Supports 12-, 24-, 48- and 96 well plate format MEAs
    https://www.axionbiosystems.com/products/MEA_Plates_Catalog
  • Built in system for electrical stimulation of the cells
  • Integrated heater and CO2 supply included in the system to allow longer measurement without interruptions
  • Axion integrated Studio –software for data acquisition and analysis

Multichannel Systems MEA2100

Simultaneous Measurements of 24 neuronal networks

Simultaneous measurement of 24 neuronal networks with 4 MEAs each having 6 separate wells. (Photo: Jonne Renvall/University of Tampere)

Features:

  • 2 measuring headstages allowing to measure 4x 60-channel MEAs simultaneously or 2x 60-channel MEAs and a 120-channel MEA simultaneously
  • Built in system for electrical stimulation of the cells
  • Phase contrast and DIC microscopy options available during measurements
  • Heater unit included in the systems to allow temperature control and monitoring
  • MC_Rack software for data acquisition
  • http://www.multichannelsystems.com/systems/mea2100-2×60-2-system
  • Compatible with Zeiss Axio Observer A1

Multichannel Systems MEA2100-lite

Features:

Multichannel Systems MEA1060-Inv

Features:

  • Microelectrode array system for electrophysiological measurements
  • Perfusion element to use perforated MEAs (pMEA) for tissue slice/retinal recordings
  • Stimulus generator for electrical and/or light stimulation of the cells
  • Dark room built for light sensitive experiments. Heater unit included in the systems to allow temperature control and monitoring
  • MC_Rack software for data acquisition
  • http://www.multichannelsystems.com/products/mea1060-inv-bc

Prices

SYSTEM RESERVATION PRICE/HOUR
Fast fluorescence imaging and patch clamp system
(Agendo name: MEAlab mainrig)
15,50 €
Patch Clamp system
(Agendo name: MEAlab patch clamp 2 setup)
15,50 €
Fast fluorescence imaging system
(Agendo name: MEAlab Imaging 2)
15,50 €
Axion Biosystems Maestro
(Agendo name: MEAlab Axion Maestro)
12 €
Multichannel Systems MEA2100
(Agendo name: MEAlab MEA2100 4xMEA)
12 €
Multichannel Systems MEA2100-lite
(Agendo name: MEAlab MEA2100-lite)
12 €
Multichannel Systems MEA1060-Inv
(Agendo name: MEAlab RetinaMEA | 3DMEA)
please inquire
the price

Pricing is valid for user groups from University of Tampere and Tampere University of Technology. Other academic or non-academic users are asked to inquire specific pricing.

Prices above are applicable from Monday to Friday 7 am to 7 pm. Discount of 50 % is applied for reservations on night time between 7 pm to 7 am and on weekends.

Tampere Facility of Electrophysiological Measurements reserves the right to price adjustments.

ACKNOWLEDGEMENT

All the users of Tampere Facility of Electrophysiological Measurements services are obligated to acknowledge the facility in publications:

“The authors acknowledge Tampere Facility of Electrophysiological Measurements for their service.”

Contacts

Reservations, equipment details: 
Juha Heikkilä
juha.heikkila(at)uta.fi
Tel: +358 40 190 1798
Room: ARVO D436

Management:
Susanna Narkilahti, Ph.D., Adj. Prof.
susanna.narkilahti(at)uta.fi
Tel: +358 40 708 5113
Room: ARVO D428

Publications

2017

Kreutzer J, Ylä-Outinen L, Mäki A-J, Ristola M, Narkilahti S, Kallio P. 2017.
Cell culture chamber with gas supply for prolonged recording of human neuronal cells on microelectrode array.
J Neurosci Methods. 2017 Mar 15;280:27-35.

Hyysalo A, Ristola M, Mäkinen M, Häyrynen S, Nykter M, Narkilahti S. 2017.
Laminin alpha 5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro.
Accepted to Stem Cell Research.

Korkka I, Johansson JK, Skottman H, Hyttinen J, Nymark S.
Characterization of chloride channels in human embryonic stem cell derived retinal pigment epithelium.
2017 EMBEC and NBC 2017 – Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. Springer Verlag, p. 454-457 4 p. (IFMBE Proceedings; vol. 65)

Välkki IA, Lenk K, Mikkonen JE, Kapucu FE, Hyttinen JAK.
Network-wide adaptive burst detection depicts neuronal activity with improved accuracy.
31 May 2017 In : Frontiers in Computational Neuroscience. 11, 40

Kapucu E, Tanskanen J, Christophe F, Mikkonen T, Hyttinen J.
Evaluation of the effective and functional connectivity estimators for microelectrode array recordings during in vitro neuronal network maturation.
14 Jun 2017 EMBEC and NBC 2017 – Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017. Springer Verlag, p. 1105-1108 (IFMBE Proceedings; vol. 65)

Böttrich M, Tanskanen JMA, Hyttinen JAK.
Lead field theory provides a powerful tool for designing microelectrode array impedance measurements for biological cell detection and observation.
26 Jun 2017 In : Biomedical Engineering Online. 16, 1, 85

Kapucu E, Välkki IA, Christophe F, Tanskanen J, Johansson J, Mikkonen T, Hyttinen J.
On electrophysiological signal complexity during biological neuronal network development and maturation.
15 Jul 2017 Proceedings of the 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

Skottman H, Muranen J, Lähdekorpi H, Pajula E, Mäkelä K, Koivusalo L, Koistinen J, Uusitalo H, Kaarniranta K, Juuti-Uusitalo K.
An in vitro outer blood-retinal barrier model based on human embryonic stem cell derived retinal pigment epithelial cells and human endothelial cells.
Experimental Cell Research, In press.

Kuusela J, Larsson K, Shah D, Prajapati C, Aalto-Setälä K.
Low extracellular potassium prolongs repolarization and evokes early afterdepolarization in human induced pluripotent stem cell-derived cardiomyocytes.
Biol Open ;6(6)777-784, 2017.

Vuorenpää H, Penttinen K, Heinonen T, Pekkanen-Mattila M, Sarkanen JR, Ylikomi T, Aalto-Setälä K.
Maturation of human pluripotent stem cell derived cardiomyocytes is improved in cardiovascular construct.
Cytotechnology ;2017.

2016

Kapucu F, Mäkinen M, Tanskanen J, Ylä-Outinen L, Narkilahti S, Hyttinen J.
Joint analysis of extracellular spike waveforms and neuronal network bursts.
J Neurosci Methods. 2016 Feb 1;259:143-55.

Lenk K, Priwitzer B, Ylä-Outinen L, Tietz LH, Narkilahti S, Hyttinen JA. (2016)
Simulation of developing human neuronal cell networks.
Biomed Eng Online. 2016 Aug 30;15(1):105.

Abu Khamidakh AE, Dos Santos FC, Skottman H, Juuti-Uusitalo K, Hyttinen J.
Semi-automatic Method for Ca2+ Imaging Data Analysis of Maturing Human Embryonic Stem Cells-Derived Retinal Pigment Epithelium.
2016 In : Annals of Biomedical Engineering. 44, 11, p. 1-13 13 p.

Kapucu FE, Mikkonen JE, Tanskanen JMA, Hyttinen JAK.
Analyzing the feasibility of time correlated spectral entropy for the assessment of neuronal synchrony.
2016 2016 IEEE 38th Annual International Conference of the Engineering in Medicine and Biology Society (EMBC). IEEE

Alarautalahti V, Ragauskas S, Holme A, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S.
Investigating retinal therapies using ex vivo retinal explants.
24 Jun 2016 In : Frontiers in Neuroscience.

Tanskanen JMA, Kapucu FE, Välkki I, Hyttinen JAK.
Automatic objective thresholding to detect neuronal action potentials.
29 Aug 2016 Proceedings of 2016 24th European Signal Processing Conference (EUSIPCO). p. 662-666 5 p.

Lenk K, Räisänen E, Hyttinen JAK.
Understanding the role of astrocytic GABA in simulated neural networks.
18 Oct 2016 2016 IEEE 38th Annual International Conference of the Engineering in Medicine and Biology Society (EMBC). IEEE, p. 6121-6124

Kapucu FE, Välkki I, Mikkonen JE, Leone C, Lenk K, Tanskanen JMA, Hyttinen JAK.
Spectral Entropy Based Neuronal Network Synchronization Analysis Based on Microelectrode Array Measurements.
18 Oct 2016 In : Frontiers in Computational Neuroscience. 10, 112

Christophe F, Andalibi V, Lenk K, Mikkonen T, Hyttinen J.
From in-silico Spiking Neural Networks to in-vitro Neural Networks.
20 Aug 2016 1 p. Proceedings of 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Orlando, Florida, USA

Tanskanen J, Kapucu FE, Hyttinen J. 2 Jul 2016.
Automatic Objective Criterion for Setting Neuronal Action Potential Spike Detection Thresholds.
10th FENS Forum of Neuroscience – Copenhagen, Denmark

Välkki I, Kapucu FE, Johansson J, Lenk K, Hyttinen J.
Characterizing Neuronal Network Burst Synchrony by Using Burst Signal.
J. 24 Jun 2016, proceedings of the 10th International Meeting on Substrate-Integrated Electrode Arrays. Reutlingen, Germany. http://dx.doi.org/10.3389/conf.fnins.2016.93.00079

Alarautalahti V, Ragauskas S, Holme A, Uusitalo-Järvinen H, Uusitalo H, Hyttinen J, Kalesnykas G, Nymark S.
Investigating retinal therapies using ex vivo retinal explants.
24 Jun 2016 In : Frontiers in Neuroscience.

Tanskanen J, Kapucu E, Välkki I, Lenk K, Hyttinen J.
Objective Thresholding of MEA Data for Action Potential Detection.
24 Jun 2016. 10th International Meeting on Substrate-Integrated Electrode Arrays – Reutlingen, Germany.  http://dx.doi.org/10.3389/conf.fnins.2016.93.00028

Kapucu FE, Vornanen I, Tanskanen J, Christophe F, Hyttinen J.
The Influence of Structural Changes and Population Interactions on the Entropy Based Synchronicity.
24 Jun 2016 2 p.10th International Meeting on Substrate-Integrated Electrode Arrays, Reutlingen, Germany http://dx.doi.org/10.3389/conf.fnins.2016.93.00053

Paavola J, Väänänen H, Larsson K, Penttinen K, Toivonen L, Kontula K, Laine M, Aalto-Setälä K, Swan H, Viitasalo M.
Slowed depolarization and irregular repolarization in catecholaminergic polymorphic ventricular tachycardia: a study from cellular Ca2+ transients and action potentials to clinical monophasic action potentials and electrocardiography.
Europace ;18(10)1599-1607, 2016.

Kuusela J, Kim J, Räsänen E, Aalto-Setälä K.
The Effects of Pharmacological Compounds on Beat Rate Variations in Human Long QT-Syndrome Cardiomyocytes.
Stem Cell Rev ;12(6)698-707, 2016.

Ojala M, Prajapati C, Pölönen RP, Rajala K, Pekkanen-Mattila M, Rasku J, Larsson K, Aalto-Setälä K.
Mutation-Specific Phenotypes in hiPSC-Derived Cardiomyocytes Carrying Either Myosin-Binding Protein C Or α-Tropomyosin Mutation for Hypertrophic Cardiomyopathy.
Stem Cells Int ;2016.

Kuusela J, Kujala VJ, Kiviaho A, Ojala M, Swan H, Kontula K, Aalto-Setälä K.
Effects of cardioactive drugs on human induced pluripotent stem cell derived long QT syndrome cardiomyocytes.
Springerplus;2016.

2015

Kapucu FE, Mikkonen JE, Tanskanen JMA, Hyttinen JAK. 2015.
Quantification and automatized adaptive detection of in vivo and in vitro neuronal bursts based on signal complexity.
2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). p. 4729-4732 4 p.

Laurila E, Ahola A, Hyttinen J, Aalto-Setälä K.
Methods for in vitro functional analysis of iPSC derived cardiomyocytes — Special focus on analyzing the mechanical beating behaviour.
2015 In : Biochimica et Biophysica Acta: Molecular Cell Research.

Vainio I, Abu Khamidakh A, Paci M, Skottman H, Juuti-Uusitalo K, Hyttinen J, Nymark S.
Computational Model of Ca2+ Wave Propagation in Human Retinal Pigment Epithelial ARPE-19 Cells.
2015 In : PLoS One. 0128434

Kapucu FE, Tanskanen JMA, Yuan Y, Hyttinen JAK.
A fast stimulability screening protocol for neuronal cultures on microelectrode arrays.
2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). p. 3440-3443 4 p

Juhola M, Penttinen K, Joutsijoki H, Varpa K, Saarikoski J, Rasku J, Siirtola H, Iltanen K, Laurikkala J, Hyyrö H, Hyttinen J, Aalto-Setälä K.
Signal analysis and classification methods for the calcium transient data of stem cell-derived cardiomyocytes.
1 Jun 2015 In : Computers in Biology and Medicine. 61, p. 1-7 7 p.

Tanskanen JMA, Kapucu FE, Hyttinen JAK.
On the threshold based neuronal spike detection, and an objective criterion for setting the threshold.
1 Jul 2015 International IEEE/EMBS Conference on Neural Engineering, NER. IEEE COMPUTER SOCIETY PRESS, p. 1016-1019 4 p.

Kiviaho AL, Ahola A, Larsson K, Penttinen K, Swan H, Pekkanen-Mattila M, Venäläinen H, Paavola K, Hyttinen J, Aalto-Setälä K.
Distinct electrophysiological and mechanical beating phenotypes of long QT syndrome type 1-specific cardiomyocytes carrying different mutations.
1 Sep 2015 In : IJC Heart and Vasculature. 8, p. 19-31 13 p.

Penttinen K, Siirtola H, Àvalos-Salguero J, Vainio T, Juhola M, Aalto-Setälä K.
Novel Analysis Software for Detecting and Classifying Ca2+ Transient Abnormalities in Stem Cell-Derived Cardiomyocytes.
PLoS One 2015 ;10(8)e0135806.

Penttinen K, Swan H, Vanninen S, Paavola J, Lahtinen AM, Kontula K, Aalto-Setälä K.
Antiarrhythmic Effects of Dantrolene in Patients with Catecholaminergic Polymorphic Ventricular Tachycardia and Replication of the Responses Using iPSC Models.
PLoS One 2015 ;10(5)e0125366.

Abu Khamidakh A, Juuti-Uusitalo K, Caetano dos Santos F, Skottman H, Hyttinen J.
Spontaneous and mechanically induced Ca2+ activity changes in hESC‐RPE cells during maturation.
1 Oct 2015 In : Acta Ophthalmologica. 93, S225

Sorkio A, Porter PJ, Juuti-Uusitalo K, Meenan BJ, Skottman H, Burke GA.
Atmospheric pressure plasma treatment increases the attachment and maturation of human pluripotent stem cell derived retinal pigment epithelial cells on biodegradable polymeric electrospun scaffolds.
Tissue Eng Part A. 2015 Sep;21(17-18):2301-14.

Sorkio A, Vuorimaa-Laukkanen E, Hakola H, Liang H, Ujula T, Valle-Delgado JJ, Österberg M, Yliperttula M, Skottman H.
Biomimetic Collagen I and IV double layer Langmuir-Schaeffer films as microenvironment for human pluripotent stem cell derived retinal pigment epithelial cells.
Biomaterials. 2015 May;51:257-69. doi: 10.1016/j.biomaterials.2015.02.005. Epub 2015 Feb 20.

Ilmarinen I, Hiidenmaa H, Kööbi P, Nymark S, Sorkio A, Wang J-H, Stanzel BV, Thieltges F, Alajuuma P, Oksala O, Kataja M, Uusitalo H, Skottman H.
Ultrathin Polyimide Membrane as Cell Carrier for Subretinal Transplantation of Human Embryonic Stem Cell Derived Retinal Pigment Epithelium.
PLoS One. 2015; 10(11): e0143669. Published online 2015 Nov 25. doi:  10.1371/journal.pone.0143669

Sorkio A, Porter PJ, Juuti-Uusitalo K, Meenan BJ, Skottman H, Burke GA.
Surface Modified Biodegradable Electrospun Membranes as a Carrier for Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.
Tissue Eng Part A. 2015 Sep;21(17-18):2301-14. doi: 10.1089/ten.TEA.2014.0640. Epub 2015 Jun 30.

2014

Ahola A, Kiviaho AL, Larsson K, Honkanen M, Aalto-Setälä K, Hyttinen J.
Video image-based analysis of single human induced pluripotent stem cell derived cardiomyocyte beating dynamics using digital image correlation.
2014 In: Biomedical Engineering Online. 13, 1, 18 p., 39

Ahola A, Pradhapan P, Laurila E, Aalto-Setälä K, Hyttinen J.
Motion Analysis Method for Determining Cardiomyocyte Beating Properties Based on Digital Image Correlation and Templates.
Computing in cardiology 2014. Computing in Cardiology, p. 1137-1140 4 p.

Tani T, Naganuma H, Harashima T, Iwagami T, Kino H, Kiyoyama K, Kellomäki M, Hyttinen J, Tanaka T.
Development of Si neural probe module with adjustable gain amplifier for neuronal signal recording.
17 Aug 2014 In : Transactions of Japanese Society for Medical and Biological Engineering. 52, p. O-377-O-378

Sasaki Y, Suzuki T, Iwagami T, Tani T, Naganuma H, Kino H, Hyttinen J, Kellomäki M,  Tanaka T.
Development of chip-surface stimulus electrode array for fully-implantable subretinal prosthesis chip.
17 Aug 2014 In : Transactions of Japanese Society for Medical and Biological Engineering. 52, p. O-253-O-254

Vuorenpää H, Ikonen L, Kujala K, Huttala O, Sarkanen JR, Ylikomi T, Aalto-Setälä K, Heinonen T.
Novel in vitro cardiovascular constructs composed of vascular-like networks and cardiomyocytes.
In Vitro Cell Dev Biol Anim 2014 ;50(4)275-86.

2013

Kiiski H, Aänismaa R, Tenhunen J, Hagman S, Ylä-Outinen L, Aho A, Yli-Hankala A, Bendel S, Skottman H, Narkilahti S.
Human cerebrospinal fluid promotes glial differentiation of human embryonic stem cell-derived neural cells but does not prevent spontaneous activity in pre-existing neuronal networks.
Biol Open. 2013 May 13;2(6):605-612

Toivonen S*, Ojala M*, Hyysalo A*, Ilmarinen T, Lundin K, Palgi J, Weltner J, Trokovic R, Rajala K, Pekkanen-Mattila M, Äänismaa R, Silvennoinen O, Skottman H, Narkilahti S, Aalto-Setälä K, Otonkoski T.
Comparative analysis of targeted differentiation of human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells reveals variability associated with incomplete transgene silencing in retrovirally derived hiPSC lines.
Stem Cells Translational Medicine. Stem Cells Transl Med. 2013 Feb;2(2):83-93.

Mäkinen M, Joki T, Ylä-Outinen L, Skottman H, Narkilahti S, Äänismaa R.
Fluorescent probes as a tool for cell population tracking in spontaneously active neural networks derived from human pluripotent stem cells.
Journal of Neuroscience Methods, J Neurosci Methods. 2013 Apr 30;215(1):88-96.

Juuti-Uusitalo K, Delporte C, Gregoire F, Perret J, Huhtala H, Savolainen V, Nymark S, Uusitalo H, Willermain F, Skottman H.
Aquaporin expression and function in human pluripotent stem cell-derived retinal pigmented epithelial cells.
Invest Ophthalmol Vis Sci, 2013 May 1;54(5):3510-9.

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